有關菠菜葉色素的濾紙色層分析結果

看板Biology (生物學)作者jasper.時間20年前 (2006/04/13 12:01)推噓0(0推 0噓 0→)

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由於9n年大考出了一題,結果與舊教材及每個學過高中生物的學生所認識不同我查了好久, 而國內大學對這類簡單的化學分析卻沒有提出答案, 任由考試引導教學內容也不妥, 至少我看到的報告是大陸--濾紙效果--胡黃ab 香港--濾紙效果--胡黃ab 美國--高中教材參考--濾紙效果--胡黃ab 其他網頁--薄層分析效果--胡ab黃只有最後一項與該年大考所示答案一致, 而解釋卻共用了極性問題來概括困擾了兩年, 在google用不同詞組搜尋, 看到這討論..摘述部份內容 "We do a chromatographic separation that sounds very much like Michael's and I thought the pigment order was chlorophyll b chlorophyll a xanthophyll 2 xanthophyll 1 (gray, "ides") and carotene at the solvent front. ... If run on a silica gel plate, I would expect the following pigments: carotenoids at solvent front, followed by a dull grey band of chlorophyll decomposition products (depending on how old the spinach prep is), followed by chlorophyll a then b then three xanthophylls (one fairly bright with the others fairly dull). ... We also do a separation of spinach in petroleum ether/ acetone on paper. The separation is based on the polarity of the pigments - least polar should migrate farthest. So at/ near the solvent front is carotene followed by xanthophyll, chl a and chl b. This order is the same as most other replies to the list. Every once in a while we get odd results. Check all the components of your set up for contamination. " http://biae.clemson.edu/biolab/spinach.html 在其中似乎也有些argue, 但大概可以見到兩分析方法有不同結果因此現在都只會教同學記跑最快的胡蘿蔔素, 其他我沒有answer 知道者其實只要將幾個band跑吸光光譜就好了, 當年若是立即有各大學立刻討論,也許不會有此困擾還好沒影響分數, 不然這帳要向誰算 -- Ξ Origin: 中興大學天樞資訊網 <bbs.nchu.edu.tw> Ξ From : 203.68.192.30