Re: [問題] anti-GST的選購問題....
※ 引述《sezko.bbs@ptt.cc (星期四去luna....)》之銘言：
> 由於訂購了一組帶有GST tag的fusion protein(Novus的)...
> 現在想定anti-GST monoclonal 的抗體....
> 可是看sigma的說明有點不太懂...
> Monoclonal anti-Glutathione-S-Transferase (GST)
> recognizes native as well as denatured-reduced forms of
> purified GST proteins, applying immunoblotting,1 dot
> blotting, and ELISA.The antibody is specific for GST
> from Schistosoma japonicum. The antibody does not
> recognize GST from rat, rabbit, porcine or bovine liver, or
> from human placenta, when tested by ELISA.
> 這意思是說......這隻抗體會hi到Schistosoma japonicum內生性的GST,
> 而不會hi到鼠,兔......人的內生性GST嗎..??
yes
> 我是要買來作western的....可是這抗體的說明沒有reference(沒有paper用過)
> 請問這抗體是不是不適用我的實驗阿....還是有推薦好用的anti-GST??
> 拜託大家..p^^q..
This one is good.
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