[問題] 問個關於restriction enzyme overdigestion的笨問題
我在fermentas的網頁上所查到關於BamHI在低鹽或PH>8的情況下會overdigestion
不過裡頭有一句話我看不懂:
Ligation and Recleavage
After 50-fold overdigestion with BamHI, more than 95% of the digested DNA
fragments can be ligated and recut.
他是就算overdigestion超過50倍也能夠進行95%以上有效率的ligation嗎??
那所謂的recut是指怎麼樣呢?
我之前曾經不小心overdigestion 1hr結果就完全ligation不出來了
有沒有對這很熟的學長姐可以幫我解惑一下?
謝謝喔...:)
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