[求救] 有人會SWISS-MODEL的Alignment Mode 功能嗎??

看板Biotech (生命科學)作者amon7279 (硬漢)時間18年前 (2008/04/23 22:23)推噓2(2推 0噓 2→)

留言4則, 3人參與討論串1/1

想請問一下這個alignment該怎麼做 http://0rz.tw/d63Wk SWISS-MODEL頁面進入後點選Alignment Mode 接著在框框輸入欲比對的Protein sequence跟Template sequence 麻煩有興趣的直接複製以下 Target Sequence(這個不用複製) >P23219|PGH1_HUMAN Prostaglandin G/H synthase 1 - Homo sapiens (Human). MSRSLLLRFLLFLLLLPPLPVLLADPGAPTPVNPCCYYPCQHQGICVRFGLDRYQCDCTR TGYSGPNCTIPGLWTWLRNSLRPSPSFTHFLLTHGRWFWEFVNATFIREMLMRLVLTVRS NLIPSPPTYNSAHDYISWESFSNVSYYTRILPSVPKDCPTPMGTKGKKQLPDAQLLARRF LLRRKFIPDPQGTNLMFAFFAQHFTHQFFKTSGKMGPGFTKALGHGVDLGHIYGDNLERQ YQLRLFKDGKLKYQVLDGEMYPPSVEEAPVLMHYPRGIPPQSQMAVGQEVFGLLPGLMLY ATLWLREHNRVCDLLKAEHPTWGDEQLFQTTRLILIGETIKIVIEEYVQQLSGYFLQLKF DPELLFGVQFQYRNRIAMEFNHLYHWHPLMPDSFKVGSQEYSYEQFLFNTSMLVDYGVEA LVDAFSRQIAGRIGGGRNMDHHILHVAVDVIRESREMRLQPFNEYRKRFGMKPYTSFQEL VGEKEMAAELEELYGDIDALEFYPGLLLEKCHPNSIFGESMIEIGAPFSLKGLLGNPICS PEYWKPSTFGGEVGFNIVKTATLKKLVCLNTKTCPYVSFRVPDASQDDGPAVERPSTEL Template sequence(這個不用複製) >gi|55670189|pdb|1U67|A Chain A, Crystal Structure Of Arachidonic Acid Bound To A Mutant Of Prostagladin H Synthase-1 That Forms Predominantly 11- Hpete. MSRQSISLRFPLLLLLLSPSPVFSADPGAPAPVNPCCYYPCQHQGICVRFGLDRYQCDCTR TGYSGPNCTIPEIWTWLRTTLRPSPSFIHFLLTHGRWLWDFVNATFIRDTLMRLVLTVRSN LIPSPPTYNIAHDYISWESFSNVSYYTRILPSVPRDCPTPMGTKGKKQLPDAEFLSRRFLL RRKFIPDPQGTNLMFAFFAQHFTHQFFKTSGKMGPGFTKALGHGVDLGHIYGDNLERQYQL RLFKDGKLKYQMLNGEVYPPSVEEAPVLMHYPRGIPPQSQMAVGQEVFGLLPGLMLYATIW LREHNRVCDLLKAEHPTWGDEQLFQTARLILIGETIKIVIEEYAQQLSGYFLQLKFDPELL FGAQFQYRNRIAMEFNQLYHFHPLMPDSFRVGPQDYSYEQFLFNTSMLVDYGVEALVDAFS RQPAGRIGGGRNIDHHILHVAVDVIKESRVLRLQPFNEYRKRFGMKPYTSFQELTGEKEMA AELEELYGDIDALEFYPGLLLEKCHPNSIFGESMIEMGAPFSLKGLLGNPICSPEYWKAST FGGEVGFNLVKTATLKKLVCLNTKTCPYVSFHVPDPRQEDRPGVERPPTEL 兩個一起都複製到框框裡接著按下submit alignment之後進入第二個頁面 Target Sequence 跟 Template Sequence 如果照上面順序放就不用重新選擇了 PDB-code 是1U67 Chain-ID我不知道所以空著再按一次submit alignment 結果卻是 Your Alignment seems incorrect.Please go BACK and correct your input. () 我試了好幾種方法也換過其他序列去比對結果都是這樣子想請問有沒有有經驗的人能跟我說我在哪個步驟錯了謝謝 -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 59.124.121.226