[求救]毛細管電泳的問題
我們實驗室原本是作藥物分析,近期想試著使用CE 分離DNA片段
我嘗試重覆他人paper, 但是無法成功
想請問是否有人有跑DNA的經驗
(我是獸醫背景,對化學和分生超級不熟啦)
我嘗試的條件如下
CE: Beckman P/ACE MDQ
Detector: 254 nm UV
Capillary: 75 um ID, uncoated fused-silica capillary, 20 cm to the detector
New condition: 1 mg/ml CTAB, 1 h
Pre-rinse: 0.5 % PEO (Mr=600000) in 100 mM TB, containing 0.36 ug/ml CTAB and 25 ug/ml EtBr ; inj. by pressure (20psi), 5 min
Sample: DNA marker (100-3000 bp); inj. by voltage (-1 kv), 12 sec
Separation: -11 kv (reverse voltage), 20 min
Rinse between runs: 0.5 M NaOH, 10 min, 20 psi; water 2 min, psi
謝謝指教
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