[求救] 有關Tricine-SDS-PAGE的Buffer。
大家好，目前我clone 一段蛋白質加fusion protein只有3.4 kDa
所以想用Tricine-SDS-PAGE來分析。
我看了一篇有關Tricine-SDS-PAGE protocol的paper有提到一段話。
http://www.nature.com/nprot/journal/v1/n1/full/nprot.2006.4.html
Mount the gels in the vertical electrophoresis apparatus (see EQUIPMENT),
and add anode buffer as the lower electrode buffer and cathode buffer as the
upper electrode buffer (anodes and cathodes are commonly marked
red and black, respectively, by the suppliers).
意思應該是指說：把SDS-PAGE電泳槽架好，然後電泳槽下面加入anode buffer，
上面則是加入cathode buffer，可是這樣子2種buffer不是會混在一起嗎? 因為我看完
整個protocol，感覺cathode buffer和anode buffer有點類似SDS-PAGE的running buffer
所以我這邊有點搞不太懂到底是怎麼一回事，
不知道各位跑Tricine-SDS-PAGE時是怎麼跑的?
還是跑電泳的時候不是加這些buffer?
謝謝^^
--
※ 發信站: 批踢踢實業坊(ptt.cc)
◆ From: 140.127.27.158
推
03/29 19:55, , 1F
03/29 19:55, 1F
推
03/29 20:29, , 2F
03/29 20:29, 2F
→
03/29 20:41, , 3F
03/29 20:41, 3F
→
03/29 20:41, , 4F
03/29 20:41, 4F
→
03/29 20:43, , 5F
03/29 20:43, 5F
推
03/29 21:10, , 6F
03/29 21:10, 6F
→
03/29 21:11, , 7F
03/29 21:11, 7F
→
03/29 21:12, , 8F
03/29 21:12, 8F
推
03/29 22:46, , 9F
03/29 22:46, 9F
→
03/29 22:47, , 10F
03/29 22:47, 10F
→
03/29 22:49, , 11F
03/29 22:49, 11F
推
03/29 23:01, , 12F
03/29 23:01, 12F
→
03/29 23:02, , 13F
03/29 23:02, 13F
→
03/29 23:02, , 14F
03/29 23:02, 14F
→
03/29 23:06, , 15F
03/29 23:06, 15F
推
03/30 00:41, , 16F
03/30 00:41, 16F
→
03/30 00:45, , 17F
03/30 00:45, 17F
推
03/30 00:50, , 18F
03/30 00:50, 18F
→
03/30 00:51, , 19F
03/30 00:51, 19F
→
03/30 11:42, , 20F
03/30 11:42, 20F
→
03/30 11:45, , 21F
03/30 11:45, 21F
→
03/30 11:45, , 22F
03/30 11:45, 22F
Biotech 近期熱門文章
PTT職涯區 即時熱門文章
12
17