[求救] IP注射劑量問題
最近開始做腹腔注射的實驗,但不是很順遂,第一次打IP(0.5ml)
結果隔天小鼠都死了,以下是我目前第二次打的劑量計算,今天觀
察小鼠感覺他們奄奄一息...我自己驗算很多次,但怕有地方是我
沒注意到的...想麻煩各位有經驗的前輩幫我檢查一下這樣的計算
是否有不對的地方...
Drug Stock: 10mg/ml
Desired dosage: 10mg/kg
(此注射的劑量為IC50的1/4,在打P針與H針的前一天都會注射一次)
For example, a mouse weighs 30g,
for 1g of mouse inject 0.01mg of drug,
hence 0.01mg x 30g=0.3mg for a 30g mouse.
To inject 0.2ml into the mouse,
you have to have a concentration of 1.5mg/ml of your drug.
1ml contains 1.5mg, hence 0.2ml contains 0.3mg.
Dilution from the drug stock of 10mg/ml to 1.5mg/ml: 10:1.5 times dilution.
For example, if you want to prepare 0.6ml of 1.5mg/ml, then add 90ul of the
stock to 510ul (DMSO) of buffer.
我的計算方式是參考：http://ppt.cc/hsKW
麻煩各位有經驗的前輩了...
謝謝
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◆ From: 140.135.60.17
※ 編輯: PriA 來自: 140.135.60.17 (11/06 13:47)
※ 編輯: PriA 來自: 140.135.60.17 (11/06 13:47)
※ 編輯: PriA 來自: 140.135.60.17 (11/06 13:49)
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11/06 16:46, , 1F
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11/06 19:04, 5F
推
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推
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11/06 23:59, 9F
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11/07 00:16, , 10F
11/07 00:16, 10F
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