[求救]stable cell line
各位大大好
我最近在建立stable cell line (使用HEK293)
transfect plasmid into cells後24hr
我換fresh的medium後(第二天)
隔天繼續讓cells長多一點(第三天)
在這幾個步驟間我都有觀察發螢光cells的比例
第二天及第三天比例都有在70%
但第四天準備要加G418篩選前
發現螢光cells的比例卻降低至20%左右
不知為什麼會這樣?
還是這是正常現象?
請各位大大能幫忙解惑
謝謝大家!
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