[求救] western blot 做膠
小弟最近要跑分子量 170kDa的蛋白
做膠時網路查到是用7.5% 但是實際操作後 發現gel很難凝固..
想請問一下有人有做過那麼大分子量的Western嗎?
可以建議配膠濃度可以給小弟嗎?
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謝謝各位!! ^^ 最後發現是APS問題
※ 編輯: xhunterx 來自: 120.126.53.153 (12/16 22:00)
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