[轉錄][分享]Promega的Guides(實驗技術指導手冊)

看板Biology (生物學)作者vixen (2tp27t)時間17年前 (2008/07/26 08:32)推噓0(0推 0噓 0→)

留言0則, 0人參與討論串1/1

在網路上可以找到很多免費的實驗技術指導手冊, 我喜歡廠商推出的手冊原因為不但它們盡力求簡顯易懂, 又涵蓋足夠的細節可以直接上手. 當然在商言商, 這類手冊裡面免不了會對自家產品宣傳, 但它免費就不計較了. 除了著名的Amersham/GE的管柱系列與Pierce的蛋白技術系列外, Promega也有一些寫得不錯的分生/生化/細生實驗技術指導手冊, 以下是由其官網複製而來,沒有pdf直接連結, 但是可以挑喜歡的前往官網看看並下載完整pdf. 這年頭併購案太多, 不先存起來哪天公司消失了都不知道.XD ======================================================================== Promega技術支援指導手冊 http://www.promega.com/guides/ (可能要先選語言才能繼續, 不論你按美國國旗或者中國國旗都是英文) 共分以下各部份, 連結裡面有每部份的大約目錄: 0.Protocols and Applications Guide (只是以下各部份的連結版, 無pdf) 1.Bioluminescent Assays for HTS article (這篇其實只是一篇paper) 2.Cloning Enzymes (Ligase,Kinase,Phosphatase) 3.DNA Analysis Notebook (PCR奧義) 4.In Vitro Protein Expression (胞內表達不靈時快來看看) 5.Nucleic Acid Purification Systems (mini/midi 抽DNA) 6.Protein Interaction (2-hybrid, IP, EMSA之類的) 7.Rabbit Reticulocyte Expression Chapter (這篇其實只是書裡的依章) 8.Restriction Enzymes Resource (限制酵素秘辛, 無pdf) 9.RNA Analysis Notebook (抽RNA與RT-PCR, Microarrays, RNAi) 10.Signal Transduction Resource (MAPK/PKA/PKB/Kinase/phosphatase信號路徑) 11.Subcloning Notebook (永遠說不完的cloning) 12.Transfection (讓真核生物表達基因的辦法) ========================================================================== http://www.promega.com/paguide/ 0.Protocols and Applications Guide 只是以下部份的連結版, 讓你網路閱讀方便, 沒有pdf. http://www.promega.com/guides/biolum_assays 1.Bioluminescent Assays for High-Throughput Screening This article, written by Keith Wood and Frank Fan, originally appeared in ASSAY and Drug Development Technologies, Volume 5, Number 1, 2007. http://www.promega.com/guides/cloning_guide 2.Cloning Enzymes The enzymes highlighted in this guide are those important in nucleic acid cloning procedures. http://www.promega.com/guides/dna_guide 3.DNA Analysis Notebook An introduction to many of the applications related to and products useful in DNA analysis and research. Revised January 2004. http://www.promega.com/guides/ive_guide 4.In Vitro Protein Expression A comprehensive guide detailing the technologies and applications associated with in vitro expression. Valuable references and schematics are also included. Promega發展方向之一,可以搭配7.同時看 http://www.promega.com/guides/nadp_guide 5.Nucleic Acid Purification Systems This guide presents many new and innovative products for use in DNA and RNA purification. Revised 9/04. http://www.promega.com/guides/protein.interactions_guide 6.Protein Interaction This guide provides an overview of common techniques used for protein interaction analysis and will assist you in determining which individual technique or combination of techniques is best suited for a particular situation/experiment. http://www.promega.com/guides/rrl.expression.systems 7.Rabbit Reticulocyte Expression Systems Chapter In the broad area of functional genomics, cell-free rabbit reticulocyte lysate (RRL) has been used extensively to elucidate the mechanisms of mammalian translation, cotranslational modifications, post-translational modifications and translocation of proteins. This chapter highlights recent functional proteomics applications that use cell-free mammalian RRL. http://www.promega.com/guides/re_guide 8.Restriction Enzymes Resource This interactive guide contains general information relevant to all restriction enzymes, procedures and applications for use and enzyme-specific technical data. Use the guide to find the best choice of enzyme for each situation. (沒有pdf, 就只是介紹而已) http://www.promega.com/guides/rna_guide 9.RNA Analysis Notebook Designed to be an introduction to many of the applications related to and products useful in RNA research. http://www.promega.com/guides/sigtrans_guide 10.Signal Transduction Resource Features overviews of key signaling pathways and the cell signaling reagents Promega offers to help unravel these complex pathways. http://www.promega.com/guides/subcloning_guide 11.Subcloning Notebook Describes procedures and reagents for use in classic and PCR subcloning, as well as for transforming bacteria and screening for recombinants. Includes a 10-page technical appendix and ordering information. http://www.promega.com/guides/transfxn_guide 12.Transfection Contains protocols, references and troubleshooting help, and features a cell line table to help you select the Promega transfection reagent that will perform best with commonly-used cell lines. -- .*#*. .*#*. .*#*. .*#*. .*#*. .*#*. | | | * * | | | * | | | * * | | | * | | | * * | | | * | | | * * | | | * * | | | * * | | | * * | | | * * | | | * * | | | * | | | * * | | | * | | | * * | | | * | | | * `*#*' `*#*' `*#*' `*#*' `*#*' `*#*' -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 128.138.171.189