Re: [問題] 有沒有較簡易的滅菌方式

看板Biology (生物學)作者Ianthegood (雜碎。)時間8年前 (2016/11/18 21:25)推噓1(1推 0噓 0→)

留言1則, 1人參與討論串2/2 (看更多)

回得有點晚這篇無法直接解決原po的問題, 不過可以提供有資源的人嘗試造福大家XD 做植物的可能有聽過一個東西叫做Plant Preservative Mixture 感覺像是fungizone的東西聽說很有效我本人是沒用過因為這東西貴到靠北但是我有估狗到這個東西的專利節錄原文如下: mixture that is optimally effective in practicing the method of the present invention for any particular plant culture medium, plant species, plant seed, plant, plant organ, plant tissue or plant cell. However, a preferred mixture of the components in the chemical agent comprises: methylchloroisothiazolinone in a concentration range of about 2.0 to about 2.6 g/l; methylisothiazolinone in a concentration range of about 0.6 to about 0.8 g/l; magnesium chloride in a concentration range of about 15.0 to about 30 g/l; and magnesium nitrate in a concentration range of about 15.0 to about 30 g/l. A most preferred mixture of the components in the chemical agent further comprises: potassium sorbate in a concentration range of about 15 to about 25 g/l; and sodium benzoate in a concentration range of about 13 to about 27 g/l. In all cases, the components of the chemical agent are mixed to form a stock solution of chemical agent using any liquid in which the components will dissolve, but preferably in water, and most preferably in distilled or deionized water. Generally, the chemical agent useful in the method of the present invention will be effective in a range of concentrations in the culture medium that allow for substantially normal seed germination or substantially normal growth or development of a plant, plant organ, plant tissue or plant cell cultured thereon. A preferred range of concentrations of the chemical agent in the culture medium is from about 0.01% to about 0.20% (v/v). A more preferred range of concentrations of the chemical agent in the culture medium is from about 0.02% to about 0.10% (v/v). The most preferred range of concentrations of the chemical agent in the culture medium is from about 0.03% to about 0.05% (v/v). 好的, 從節錄的文章裡我們可以看到最重要的幾個成分是: methylchloroisothiazolinone methylisothiazolinone sodium benzoate potassium sorbate 剩下的一些大概都是沒什麼意思的鹽類好這幾個東西(尤其前兩項)都是已知劑量過高可能會對人體皮膚造成過敏反應, 要單買其實也很不容易. 但是有些東西裡面常常會有加幾項, 例如洗面乳, 洗髮乳, 潤髮乳之類的又但是所有"洗"類的裡面又有高劑量的surfectant, 細胞大概也很難忍受所以, 個人覺得真的最可能實行的就是「潤髮乳」然後我的確是有在某個牌子的潤髮乳裡面看到全部這些東西, 不過還沒有時間跟資源來嘗試, 現在把這個資訊pass on給大家看有沒有勇者可以試試看XD -- ※ 發信站: 批踢踢實業坊(ptt.cc), 來自: 131.111.5.164 ※ 文章網址: https://www.ptt.cc/bbs/Biology/M.1479475512.A.F0E.html