[問題] 第一次看PAPER 請各位大大幫幫我

看板Biotech (生命科學)作者flywww (髒髒)時間19年前 (2006/12/03 14:34)推噓3(3推 0噓 2→)

留言5則, 3人參與討論串1/1

不知道在這裡可不可以問這些問題這是我第一次讀paper 然後要報告小弟程度真的不是很好遇到很多問題不知道有沒有大大可以幫我解答感機不盡>"< 篇名是difution characterist of collagen film <原文>Collagen films prepared by treating collagen gel solutions with different concentrations of glutaraldehyde were evaluated as a biodegradable and biocompatible drug carrier for cosmetically effective agents in this study. 在這個論文裡論述膠原蛋白膠體溶液再不同濃度的戊二酸醛裡討論藥物傳輸的生物降解性與生物相容性(這一段是摘要的 cosmetically這個字真的就不知道怎麼翻) Then the gel solutions were put into a jacketed container to measure the viscosity. A circulating water bath was connected to this jacketed container for maintaining the temperature of the container at 258C. *jacketed container 是什麼樣的容器 *circulating water bath是什麼維持溫度的系統!? 水蒸氣嗎? <原文> a suitable spindle was mounted and activated with a desired rotating speed,and measurements were taken after the designated temperature was reached and a stable reading was recorded. The viscosity was recorded at predetermined time intervals. An average of three replicates was reported for each time point. *spindle這是什麼形狀實驗步驟有點看不懂是先把它轉成薄膜在量他的黏度嗎? <原文>A collagen solution at 1% (w/w) was prepared by dissolving collagen in 3% acetic acid. Then various concentrations of glutaraldehyde (0, 0.05, 0.075, 0.1, 0.2, 0.25, and 0.3%, v/w) were added, and these mixtures were immediately transferred into a round acrylic mold, which had been sealed with parafilm paper (American Can, USA) around the bottom.These molds were then placed in an electric dryer(Intech, Osaka, Japan) until the weight of the collagen film approached a constant value. Then, the parafilm paper was carefully peeled off, and the collagen films were stored in a desiccator at ambient temperature until use. The thickness of the collagen films was measured at five different randomly selected places. The uniformity of the thickness of collagen films was acceptable since the coefficient of variation was less than 5%. round acrylic mold是類似培養皿的樣子嗎? <翻譯>把膠原蛋白溶在3%的醋酸溶液裡配成1%的膠原蛋白溶液並加入(0 0.05 0.075 0.1 0.2 0.25 0.3v/w) 的戊二酸醛並直接加入圓形丙烯酸的模子裡並用石蠟紙密封並放置在電子乾燥器裡直到膠原蛋白膜的重量幾乎不變在把時蠟紙小心的撕掉並把膠原蛋白儲藏在乾燥溫度的環境將厚的膠原蛋白膜在隨機不同的地方量測 The uniformity of the thickness of collagen films was acceptable since the coefficient of variation was less than 5%. 這句就不大懂 <原文> Diffusion studies were carried out using retinoic acid (0.01%), retinol palmitate (0.1%), ascorbic acid 6-palmitate (0.1%), and tocopherol acetate (0.1%) as model drugs either in solution or a gel form (using1%, w/w, collagen as a gelling agent and 10%Cremophor RH40 as a solubilizer) at 378C, in a flow-through diffusion system. This system consists of a multi-channel peristaltic pump (202U/AA, Watson Marlow), a fraction collector (Retriever IV,ISCO, USA), a circulating water bath, and six units of flow-through diffusion cells. The flow-through diffusion cells contain two side arms, which enable the conduction of receiver-cell media from a peristaltic pump to a fraction collector. The temperature was maintained at 378C by circulating constant temperature water through the outer jacket of the receiver cell. The surface area of the receiver cell opening was 1.77 cm , and the volumes for the donor and receiver compartments were 0.8 and 0.1ml, respectively. A 0.9% normal saline solution (containing 0.01% sodium azide and 10% Cremophor RH40) was used as the receiver cell medium. The receiver cell media were stirred at 450 rpm by externally driven, teflon-coated magnetic bars. Collagen films prepared with different concentrations of glutaraldehyde were mounted onto each receiver cell, and an O-ring and cell top were placed on the top of each membrane. These com- ponents were then clamped securely in place. The receiver cell medium reservoir was maintained at 378C. Subsequently, a solution or gel form of the model drugs was applied on the top of each collagen film. All samples were collected over 10-h periods. The diffusion of model drugs through collagen films was followed by determining their concentrations in the collected medium as a function of time. The concentrations of these model drugs were analyzed by a validated HPLC method as described below. 1.multi-channel peristaltic pump (202U/AA,Watson Marlow), a fraction collector (Retriever IV,ISCO, USA), a circulating water bath (這些是怎樣的儀器!?) 2.因為很多儀器都沒有使用過所以真的不懂他的實驗流程可能要麻煩大大解說一下不用翻譯大概說明一下他的實驗流程好長一篇怕沒有人回很快就要報告了真的需要大家幫我也希望給我一些意見對於以後做專題還有讀paper 感謝各位大大 -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 218.160.80.176