[求救] 請問30%的acrylamide改成40%會有什麼問題嗎!?
不好意思..
我想請問一下..
如果本來都是用30%的acrylamide改成40%的..
跑出來的結果會有什麼差嗎?!?!
我查了一下資料還有問幾個學長..他們說其實主要就是孔逕變小..
然後如果跑同樣sample時間就拉長..
因為目前學弟妹跑的都是55KD的SAMPLE..他們跑出來有點問題..
但是是同時跑的膠一片很好..一片卻有點醜!!
不知道這樣會影響嗎?!
我是覺得應該是LOAD膠的時候出問題,導致膠的構形改變才會這樣..
而且我最近跑43KD的ACTIN沒有問題耶!!!
兩片都跑的很好...
所以希望能請板友幫我解釋一下囉!!!
謝謝!!!!!!!!!
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