[求救] glycerol stock 若是被雜菌污染
看板Biotech (生命科學)作者allthebest (allthebest)時間12年前 (2013/05/04 23:46)推噓3(3推 0噓 20→)留言23則, 6人參與討論串1/2 (看更多)
事由:plasmid transfection細胞後一直污染
做法:stock 種midi 然後抽plasmid
plasmid+jetpei+transfers buffer 做transfection(24hr觀察就有長菌了)
已重抽兩遍....做transform都有污染...
已做的truble shooting:
1.2.3.buffer,plasmid,jetpei only
4.plasmid+buffer+jetpei
結果1，2，3乾淨4又長菌了(我要崩潰了)到底為什麼啦？(爆氣
尤其是我一次抽很多不同midi 只有這一隻plasmid怪怪的 總之我決定重新transform
不過先假設是我眼殘(沒在plasmid only看到有菌)真的是plasmid有污染好了 我很好奇如果在stock有雜菌在抽完plasmid後也會有菌菌污染嗎？(我很確定elution buffer是乾淨的)但就算stock有雜菌我最後不是也只會得到雜菌的plasmid嗎？？？
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