[求救] western blot抗體問題
請問各位，這幾次壓片都沒有結果。
抗體是用Santa cruz的,都存放於4度C冰箱
都用PBST配1:1000，壓O/N
可是抗體是學姊留下來的，學姊大概一年前畢業吧
二抗也是用PBST配的，1:5000
每次去壓的結果都頗醜．
p27和p21根本沒出現過...只有出現actin
而且actin也不在43這個位置上....
想詢問一下各位怎麼解決...換抗體嗎還是要用脫脂牛奶去配抗體?
實驗室沒半個學長姐超級囧欸...
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