[求救] 蛋白質表現出來做成抗體染IHC的問題
大家好
最近收到之前 把表現出來的蛋白質給抗體公司 做成的抗體
拿來試染
我用的是Biogenex的系統 用DAB呈色
先拿PCR陰性的片子來試染
把原液稀釋5000X 10000X 25000X 50000X來試染
看背景有沒有上
想不到染出來,整片有組織的地方都上了..又稠又濃
根本無法辨認
請問我下一步 該怎麼trouble shooting..
因為失敗所以還有一個疑問:
我自己生產出來的一抗, 可以接上comerical kit的二抗嗎
謝謝
--
※ 發信站: 批踢踢實業坊(ptt.cc), 來自: 140.112.96.58
※ 文章網址: http://www.ptt.cc/bbs/Biotech/M.1418282975.A.9E7.html
→
12/11 20:11, , 1F
12/11 20:11, 1F
推
12/11 20:21, , 2F
12/11 20:21, 2F
推
12/11 22:01, , 3F
12/11 22:01, 3F
→
12/11 22:03, , 4F
12/11 22:03, 4F
→
12/11 22:05, , 5F
12/11 22:05, 5F
→
12/11 22:06, , 6F
12/11 22:06, 6F
→
12/11 22:06, , 7F
12/11 22:06, 7F
→
12/11 23:11, , 8F
12/11 23:11, 8F
→
12/12 18:04, , 9F
12/12 18:04, 9F
→
12/12 18:05, , 10F
12/12 18:05, 10F
→
12/12 18:06, , 11F
12/12 18:06, 11F
→
12/12 20:27, , 12F
12/12 20:27, 12F
→
12/12 20:27, , 13F
12/12 20:27, 13F
→
12/12 20:27, , 14F
12/12 20:27, 14F
→
12/12 20:27, , 15F
12/12 20:27, 15F
推
12/12 22:03, , 16F
12/12 22:03, 16F
→
12/12 22:04, , 17F
12/12 22:04, 17F
推
12/12 22:05, , 18F
12/12 22:05, 18F
→
12/13 04:03, , 19F
12/13 04:03, 19F
→
12/13 04:03, , 20F
12/13 04:03, 20F
→
12/13 04:05, , 21F
12/13 04:05, 21F
→
12/13 04:06, , 22F
12/13 04:06, 22F
→
12/13 04:06, , 23F
12/13 04:06, 23F
→
12/13 04:09, , 24F
12/13 04:09, 24F
→
12/13 04:09, , 25F
12/13 04:09, 25F
Biotech 近期熱門文章
PTT職涯區 即時熱門文章