[方法] 直接vortex cell pellet?
在某個染色實驗protocol裡看到一個步驟有點陌生
~~
10. Add 2 mL of 1X assay buffer to each tube.
11. Mix each tube.
12. Centrifuge the stained cells at < 200 X g for 5 minutes at RT.
13. Carefully remove and discard supernatants.
14. Gently vortex the pellets to disrupt any cell-to-cell clumping.
^^^^^^^^^^^^^^^^^^
15. Resuspend the cells in 1 mL of 1X assay buffer.
~~
如果不是我理解錯誤，
它應該是叫我直接vortex cell pellet without buffer沒錯吧？
因為我做了4年多的細胞實驗，
好像沒有做過直接vortex cell pellet的經驗
這樣的步驟合理嗎？ 還是它純粹是step.14 &15寫顛倒？
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※ 編輯: ararthur (140.112.121.122), 03/17/2015 15:20:22
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