[求救] cDNA取量問題(Realtime PCR)
這問題可能有點基礎
不過小弟第一次做realtime PCR
因此還是發文請教了...
RNA轉cDNA步驟:
假設我有A，B兩個sample
A sample: 1ug RNA要取0.38 ul
B sample: 1ug RNA要取0.72 ul
轉cDNA的時候我就取A sample 0.38ul和B sample 0.72ul
也就是各取1ug的RNA去轉cDNA
這樣我要上機做Realtime PCR之前
cDNA要再做一次定量嗎?
還是我A sample和B sample的cDNA不用定量，直接取1ul就會取到相同的量?
請版友不吝指教了 謝謝!
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