[閒聊] 詢問大腸桿菌BL21(DE3)表達重組蛋白
問題是這樣的蛋白預計分子量為38kDa.(不含6x his )
但實際跑SDS-PAGE
目標片段跟marker比對。卻是介於40-55kDa..
請問該如何解呢
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