[求救] 4%PFA固定細胞問題
想請問各位做過ICC的大大們，
事情是這樣的，我們老師要求我在做ICC之前先試試用PFA固定細胞(在tube裡)，然後上Flow cytometry來確認PFA固定會不會影響到抗體的binding，所以我剛才就試著用上flow的方式來固定細胞，但是在PBS wash兩次之後細胞就消失了，根本還來不及stop
請問用PFA固定細胞是不是一定要貼片的細胞呢？？我不知道問題出在哪裡QQ
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