[問題] 請幫忙翻譯..消失

看板Biology (生物學)作者Mu6v2時間18年前 (2007/10/05 20:39)推噓3(3推 0噓 2→)

留言5則, 3人參與討論串1/2 (看更多)

瞬時轉染和雙螢光素?分析 (打不出來一直出現問號= =) Transient transfections and dual luciferase reporter assays T47D cells were subcultured with 0.56x10 5/well cells in 24-well plates 1 day before transfection. 這段是說T47D乳癌細胞株再轉染前先次培養在24well的plate1天,每個well要有0.5x10 5? Fugene 6 was used to transfect T47D cells with both 0.19 mg/well of a plasmid containing Firefly-LUC under the regulation of three copies of HRE from the PGK-1 gene (OB-HRE) and a minimal Simian virus 40 (SV40) promoter and 0.01 mg/well of pRL SV40 (which contains an SV40-driven Renilla LUC construct) as an internal control to normalise the Firefly-LUC expression according to the manufacturer’s instructions. 根據廠商的指示3個HRE的複製品:PGK-1 gene,最小的SV40啟動子和0.01mg/well的pRL SV4 當作實驗室內部control去 ????? Firefly-LUC的表現以fugene6轉染T47D乳癌株細胞,plasmid嵌入Firefly-LUC Following 12 h transfection and 24 h serum starvation, cells were either incubated in the presence or absence of bFGF (10 ng/mL) for 6, 18, or 24 h under normoxic or hypoxic conditions, respectively, or pretreated with PD98059 (20 mM) or LY294002 (20 mM) for 20min before exposure to hypoxia in the presence or absence of bFGF (10 ng/mL) for 18 h. 接著12小時的轉染和24小時的serum starvation,在有氧或缺氧的情況下細胞培養存在或缺乏bFGF 6,18,24小時在暴露在缺氧再存在或缺乏bFGF前以PD98059或LY294002前處理20分 Whole cell lysates were collected and a luciferase assay was performed using the luciferase assay system from Promega following the manufacturer’s procedure. Results were quantified with a MicroBeta luminescence counter. 最後一段翻譯到氣餒了.......... 對不起..我真的不行了看不懂啦..以前沒學過過也沒有跟實驗上網查知到了轉染是將DNA送進細胞的方法但實在沒辦法將文章跟轉染連結在一起...請看的懂得人幫忙翻一下..感激不盡 -- ※ 發信站: 批踢踢實業坊(ptt.cc)