qPCR NEWS September 2012 - focus on CNA (circulating nucleic aci

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qPCR NEWS September 2012 - focus on CNA (circulating nucleic acids) ---------------------------------------------------------------------------= -- Dear researcher, dear Gene Quantification page reader, Our newsletter informs about the latest news in quantitative real-time PCR = (qPCR and RT-qPCR), which are compiled and summarised on the=20 Gene Quantification homepage. The focus of this newsletter issue is: * UPDATE of various new papers - CNA (circulating nucleic acids) - http://= CNA.gene-quantification.info * Second announcement qPCR & NGS Symposium in March 2013 - http://www.qPCR-= NGS-2013.net=20 * GenEx - a powerful tool for qPCR data analysis - download a free trial ve= rsion - http://GenEx.gene-quantification.info ---------------------------------------------------------------------------= -- If this newsletter is not displayed correctly by your email client, please = use following http://qPCRnews.gene-quantification.info ---------------------------------------------------------------------------= -- UPDATE of various new papers - CNA (circulating nucleic acids) - http://cn= a.gene-quantification.info Most of the DNA and RNA in the body is located within cells, but a small am= ount of nucleic acids can also be found circulating freely in the blood. Th= ese DNA, RNA and small RNA molecules are thought to come from dying cells t= hat release their contents into the blood as they break down. The term "Cir= culating Nucleic Acids =3DCNA" refers to segments of DNA or RNA found in th= e bloodstream. CNAs offers a non-invasive approach to a wide range in diagnostics of clini= cal disorders that will allow the basic information necessary not only for = use in predictive medicine but also for direct use in acute medicine. Furth= er free CNAs offer unique opportunities for early diagnosis of clinical con= ditions, e.g. in early cancer detection via microRNAs. ---------------------------------------------------------------------------= -- Circulating Nucleic Acids - new papers http://CNA.gene-quantification.info Genomic analysis of fetal nucleic acids in maternal blood Dennis Lo YM, Kwun Chiu RW. Annu Rev Genomics Hum Genet. 2012 Sep 22;13: 285-306 Nucleic acids in circulation: are they harmful to the host? Mittra I, Nair NK, Mishra PK. J Biosci. 2012 Jun;37(2): 301-312 Non-invasive prenatal diagnosis of single-gene disorders from maternal bloo= d Bustamante-Aragon=E9s A, Rodr=EDguez de Alba M, Perlado S, Trujillo-Tiebas = MJ, Arranz JP, D=EDaz-Recasens J, Troyano-Luque J, Ramos C. Gene. 2012 Aug 1;504(1): 144-149 Circulating micro-RNAs as potential blood-based markers for early stage bre= ast cancer detection Schrauder MG, Strick R, Schulz-Wendtland R, Strissel PL, Kahmann L, Loehber= g CR, Lux MP, Jud SM, Hartmann A, Hein A, Bayer CM, Bani MR, Richter S, Ada= mietz BR, Wenkel E, Rauh C, Beckmann MW, Fasching PA. PLoS One. 2012;7(1): e29770 Evaluation of circulating tumor cells and circulating tumor DNA in non-smal= l cell lung cancer: association with clinical endpoints in a phase II clini= cal trial of pertuzumab and erlotinib Punnoose EA, Atwal S, Liu W, Raja R, Fine BM, Hughes BG, Hicks RJ, Hampton = GM, Amler LC, Pirzkall A, Lackner MR. Clin Cancer Res. 2012 Apr 15;18(8): 2391-2401 De novo sequencing of circulating miRNAs identifies novel markers predictin= g clinical outcome of locally advanced breast cancer Wu X, Somlo G, Yu Y, Palomares MR, Li AX, Zhou W, Chow A, Yen Y, Rossi JJ, = Gao H, Wang J, Yuan YC, Frankel P, Li S, Ashing-Giwa KT, Sun G, Wang Y, Smi= th R, Robinson K, Ren X, Wang SE. J Transl Med. 2012 10:42. Genome-wide maps of circulating miRNA biomarkers for ulcerative colitis Duttagupta R, DiRienzo S, Jiang R, Bowers J, Gollub J, Kao J, Kearney K, Ru= dolph D, Dawany NB, Showe MK, Stamato T, Getts RC, Jones KW. PLoS One. 2012;7(2): e31241 Circulating microRNAs in plasma of patients with gastric cancers Tsujiura M, Ichikawa D, Komatsu S, Shiozaki A, Takeshita H, Kosuga T, Konis= hi H, Morimura R, Deguchi K, Fujiwara H, Okamoto K, Otsuji E. Br J Cancer. 2010 Mar 30;102(7): 1174-1179 Exosomes: Fit to deliver small RNA Zomer A, Vendrig T, Hopmans ES, van Eijndhoven M, Middeldorp JM, Pegtel DM. Department of Pathology; Cancer Center Amsterdam; VU University Medical Cen= ter; Amsterdam, The Netherlands. Commun Integr Biol. 2010 Sep;3(5): 447-450 The majority of microRNAs detectable in serum and saliva is concentrated in= exosomes Gallo A, Tandon M, Alevizos I, Illei GG. PLoS One. 2012;7(3):e30679 Mechanism of transfer of functional microRNAs between mouse dendritic cells= via exosomes Montecalvo A, Larregina AT, Shufesky WJ, Stolz DB, Sullivan ML, Karlsson JM= , Baty CJ, Gibson GA, Erdos G, Wang Z, Milosevic J, Tkacheva OA, Divito SJ,= Jordan R, Lyons-Weiler J, Watkins SC, Morelli AE. Blood. 2012 Jan 19;119(3): 756-766 Functional transfer of microRNA by exosomes Stoorvogel W. Blood. 2012 Jan 19;119(3): 646-648 Comment on -- Mechanism of transfer of functional microRNAs between mouse d= endritic cells via exosomes [Blood. 2012] Secretory microRNAs as a versatile communication tool Iguchi H, Kosaka N, Ochiya T. Commun Integr Biol. 2010 Sep;3(5): 478-481 ---------------------------------------------------------------------------= -- Second announcement qPCR & NGS Symposium in Freising-Weihenstephan 18-22 March 2013 http://www.qPCR-NGS-2013.net Download the latest Event Flyer =3D> http://tinyurl.com/qPCR-NGS-2013 On behalf of the Organisation Committee and the Scientific Board it is a gr= eat pleasure to invite you to the 6th International qPCR & NGS 2013 Event. = The event is divided in a 3-day scientific Symposium with an Industrial Exh= ibition and various 2-day Application Workshop to be held at the Center of = Life Science in Freising Weihenstephan, Technische Universit=E4t M=FCnchen = (Germany). The great international interest in the previous meetings ( qPCR= 2004 to qPCR 2011 ) led us to the decision to repeat the Symposium in spri= ng 2013. We expect 500-600 participants coming from all over the world, in = 2011 we could welcome participants from 56 contries, and roughly 30-40 inte= rnational companies in the qPCR Industrial Exhibition. We have set the date for the qPCR & NGS 2013 Event to 18th - 22nd March 201= 3. The event location is the central lecture hall complex and the foyer at = TUM (Technical University of Munich) in Freising Weihenstephan, Germany (Go= ogle Maps link or Google Earth link). The TUM and the Biotech region around= Munich is part of the largest Biotech cluster in Europe, located close to = the Munich airport in the heart of Bavaria. The focus of the qPCR & NGS 2013 Event will be on: Next Generation Thinki= ng in Molecular Diagnostics Leading academic researchers and industrial contributors in the field will = participate in the symposium, which will be an arena for fruitful discussio= ns between researchers of different backgrounds. The Symposium Talks, Poste= r Sessions, Industrial Exhibition and associated qPCR & NGS Application Wor= kshops offer an overview of the present knowledge and future developments i= n qPCR, next generation sequencing and gene expression measurement technolo= gy and its wide applications in research. The symposium will focus on 70 lectures and a huge poster exhibition will b= e presented by internationally recognised experts in their field. The empha= sis will be on unbiased, didactic information exchange. Internationally ren= own speakers will be participating in a lively and exciting programme enabl= ing the valuable exchange of information in the qPCR and Next Generation Se= quencing field. One third of the talks will be presented by selected invite= d speakers, one third will be selected from the submitted abstracts and one= third will be presented by qPCR & NGS related company R&D representatives.= All scientific contributions will be published in the Symposium Proceeding= s (ISBN to be announced). Full papers from selected invited academic and industrial speakers and appl= ication notes from industrial speakers will be published in a METHODS speci= al issue =93Transcriptional Biomarkers=94 edited by Michael W. Pfaffl (pub= lished January 2013). At the event all participants will receive a free pr= int copy of this special Methods issue. Please have a look to our previous = issue =3D> =93The ongoing evolution of qPCR=94 METHODS special qPCR Vol 50= issue 4 (April 2010) Please register using the Internet based ConfTool registration and submissi= on platform =3D> http://registration.qPCR-NGS-2013.net ---------------------------------------------------------------------------= -- Symposium Talk and Poster sessions: http://sessions.qPCR-NGS-2013.net - Main Topic: Molecular diagnostics - Main Topic: Next Generation Sequencing (NGS) - Main Topic: Transcriptional Biomarkers - High throughput analysis in qPCR - Systems biology - Single-cells diagnostics - MIQE & QM strategies in qPCR - non-coding RNAs - microRNA, siRNA, long non-coding RNAs - Digital PCR & Nano-fluidics - Pre-analytical Steps - BioStatistics & BioInformatics - qPCR & NGS data analysis - Lunch Seminars: - qBASEplus - data analysis lunch seminar - GenEx - data analysis lunch seminar - NGS data analysis lunch seminars - more to be announced........ View our qPCR 2011 event trailer on YouTube =3D> http://www.youtube.com/wat= ch?v=3Dcp8WwPyLW8Y Download the latest Event Flyer =3D> http://tinyurl.com/qPCR-NGS-2013 Please register using the Internet based ConfTool registration and submissi= on platform =3D> http://registration.qPCR-NGS-2013.net ---------------------------------------------------------------------------= -- GenEx 5 - A Powerful Tool For qPCR Data Analysis Download a free trail version here =3D> http://GenEx.gene-quantification.in= fo GenEx is a popular software for qPCR data processing and analysis. Built in= a modular fashion GenEx provides a multitude of functionalities for the qP= CR community, ranging from basic data editing and management to advanced cu= tting-edge data analysis. View our webpage =3D> http://GenEx.gene-quantific= ation.info Basic data editing and management Arguably the most important part of qPCR experiments is to pre-process the = raw data into shape for subsequent statistical analyses. The pre-processing= steps need to be performed consistently in correct order and with confiden= ce. GenEx Standard=92s streamlined and user-friendly interface ensures mist= ake-free data handling. Intuitive and powerful presentation tools allow pro= fessional illustrations of even the most complex experimental designs. Advanced cutting-edge data analysis When you need more advanced analyses GenEx Enterprise is the product for yo= u. Powerful enough to demonstrate feasibility it often proves sufficient fo= r most users demands. Current features include parametric and non-parametri= c statistical tests, Principal Component Analysis, and Artificial Neural Ne= tworks. New features are continuously added to GenEx with close attention t= o customers=92 needs. New features Sample handling and samples individual biology often contribute to confound= ing experimental variability. By using the new nested ANOVA feature in GenE= x version 5 user will be able to evaluate variance contributions from each = step in the experimental procedure. With a good knowledge of the variance c= ontributions, an appropriate distribution of experimental replicates can be= selected to minimize confounding variance and maximize the power of the ex= perimental design! For experiments with complex features, such as for examp= le multifactorial diseases, analytical relationships and classifications ma= y not readily be available. The support vector machine feature in the new v= ersion of GenEx is so easy to use that it will make this advanced supervise= d classification method easily available to novice users, while providing a= ccess to advanced parameters for experts. Download a free trail version here =3D> http://GenEx.gene-quantification.in= fo GenEx PDF user guides: * GenEx user guide=20 * GenEx user guide - Exiqon Wizard=20 * GenEx user guide - Roche Wizard ---------------------------------------------------------------------------= -- Please forward this qPCR NEWS http://api.addthis.com/oexchange/0.8/forward= /email/offer?url=3Dhttp://qPCRnews.gene-quantification.info&title=3DJoin+ou= r+monthly+newsletter+on&username=3DqPCR-NEWS&email_template=3D&lng=3Den-us = to further scientists and friends who are interested in qPCR ! Best regards, Michael W. Pfaffl responsible Editor of the Gene Quantification Pages If this newsletter is not displayed correctly by your email client, please = use following=20 LINK http://qPCRnews.gene-quantification.info/ ---------------------------------------------------------------------------= -- The qPCR NEWS and the Gene Quantification Pages are educational sites with = the only purpose of facilitating access to qPCR related information on the = internet. The qPCR NEWS and the Gene Quantification Pages are edited by Mic= hael W. Pfaffl. Copyright =A92005-2012 All rights reserved. Any unauthorized use, reproduct= ion, or transfer of this message or its contents, in any medium, is strictl= y prohibited. 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