Dear Taiwan Fellows, pls judge yourself
看板Biotech (生命科學)作者mitbbsuser (mitbbs user)時間19年前 (2006/12/17 01:17)推噓0(0推 0噓 0→)留言0則, 0人參與討論串1/8 (看更多)
Beware: some images are huge and IE or other explorers might zoom out them
automatically. For the best result, pls view at 100% size. Thank you.
The original Figure2C published on Cell with illustrations
http://capa.zoto.com/img/original/c910fb371829d67b09568e6cb247292d-.jpg

Then here comes the photoshop difference blending results:
http://capa.zoto.com/img/original/50d0d142ac7730621cfb559c2080c14b-.jpg



Then here comes the so called repeat figure2C-1, which you could download
yourself through the link lnalna posted.
http://capa.zoto.com/img/original/f13297b8b79dc41a1a42b9c57330fa3a-.jpg

then a little photoshop adjustment like this:
http://capa.zoto.com/img/original/a531df68a7ec7a0a46091555b5cbda00-.jpg

here is the final result:
http://capa.zoto.com/img/original/1aebae3ec7ba8b874ae6aaf935ba596b-.jpg

Some people also use Matlab Corr(2) calculation and ImageJ Frourier Transform
to analyse the original figure2C published on Cell and got the same conclusion.
I won't make any conclusion here. Judge yourself with your eyes.
btw, the methodology shown here is exactly the same method which the top
journals use, like JCB.
If you want to argue about "false positive" due to the image compression.
You'd better not. The original images are 500dpi, 8bit, grayscale TIFF.
and JPEG compression could only give you some 8*8 squares. Here is a control
from another Gel done by the other lab.
http://capa.zoto.com/img/original/98feb861409c0c300478321085f28812-.jpg

although the bands are quite similar between different lanes, the background
patterns are obviously different.
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