Re: [求救] 請問virus infection時加入的polybrene
http://pingu.salk.edu/~sefton/Hyper_protocols/infectRetro.html
這邊有提到一些~~~~
Infection with retroviruses is facilitated greatly by polybrene.
This is a small, positively charged molecule that binds to cell
surfaces and neutralizes surface charge.
This apparently allows the viral glycoproteins to bind more
efficiently to their receptors, because it reduces the repulsion
between sialic acid-containing molecules.
To use polybrene optimally, the cells to be infected should
be pre-treated with polybrene and the virus adsorption should
be done in the presence of polybrene.
※ 引述《momyourb (不死無敵小強)》之銘言：
: 請問各位，我在virus infection的protocol上看到
: 需要加入polybrene，上網查了一下大概是可以增加
: 病毒顆粒對細胞的表面結合能力，但是其機制是什麼啊？
: 有更詳細的說明嗎？
: 另外請問一下polybrene哪家有在賣啊？
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