Re: [求救] 抽RNA的器具要不要滅菌?
http://www.ambion.com/techlib/tb/tb_178.html
滅菌還是會有效果的
塑料器具要用NaOH處理 配藥的水要用DEPC處理過後滅菌
實驗區域盡量少有人走動 滅菌鍋也要記得清理和檢查
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09/10 22:58, , 1F
09/10 22:58, 1F
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09/10 22:58, , 2F
09/10 22:58, 2F
1. Autoclaving is not effective at eliminating RNase in solution because the
RNases simply renature as the solution cools.
"FALSE", but... Autoclaving alone does indeed inactivate a substantial amount
of RNase A (Figure 1). Various concentrations of RNase A were added to PBS
and autoclaved. Aliquots of each solution were mixed with a 304 base
32P-labeled RNA probe and incubated at 37°C for one hour, followed by
electrophoresis and exposure to film. Without autoclaving, the probe begins
to degrade at an RNase concentration of 100 pg/ml. Autoclaving inactivates
enough of the RNase A to protect the probe from degradation up to a
concentration of 1 μg/ml. Note that only a portion of the RNase is
inactivated by autoclaving, otherwise the RNA probe would remain intact at
any RNase concentration. Autoclaving alone may be sufficient to eliminate
enough RNase for some applications. However, since neither the extent of
RNase contamination nor at what RNase concentration the assay is sensitive is
known, DEPC should be used as an added precaution. Also note that these
experiments were only performed on RNase A and may not hold true for other
RNases.
※ 編輯: saviora 來自: 124.11.149.96 (09/10 23:54)
推
09/11 01:02, , 3F
09/11 01:02, 3F
不好意思
※ 編輯: saviora 來自: 124.11.149.96 (09/11 01:20)
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