[-癌-] miRNAs in lung cancer

看板Medicine (醫學生物)作者biocommander (世翔)時間15年前 (2010/04/13 10:35)推噓1(1推 0噓 1→)

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第一次PO文不知道該PO在哪個版好不過想和各位板友分享文章若有不妥或想討論者請來信感恩~~ ^^" miRNAs in lung cancer - Studying complex fingerprints in patient's blood cells by microarray experiments Andreas Keller, Petra Leidinger, Anne Borries1, Anke Wendschlag1, Frank Wucherpfennig(1), Matthias Scheffler(1), Hanno Huwer(3), Hans-Peter Lenhof(4) and Eckart Meese(2) (1) febit biomed gmbh, Im Neuenheimer Feld 519, 69120 Heidelberg, Germany (2) Department of Human Genetics, Medical School, Saarland University, Building 60, 66421 Homburg, Germany (3) Department of Cardiothoracic Surgery, Voelklingen Heart Center, 66333 Voelklingen, Germany (4) Center for Bioinformatics, Saarland University, Building E11, 66041 Saarbruecken, Germany BMC Cancer 2009, 9:353doi:10.1186/1471-2407-9-353 Abstract Published: 6 October 2009 ______________________________________________________________________________ Background Deregulated miRNAs are found in cancer cells and recently in blood cells of cancer patients. Due to their inherent stability miRNAs may offer themselves for blood based tumor diagnosis. Here we addressed the question whether there is a sufficient number of miRNAs deregulated in blood cells of cancer patients to be able to distinguish between cancer patients and controls. Methods We synthesized 866 human miRNAs and miRNA star sequences as annotated in the Sanger miRBase onto a microarray designed by febit biomed gmbh. Using the fully automated Geniom Real Time Analyzer platform, we analyzed the miRNA expression in 17 blood cell samples of patients with non-small cell lung carcinomas(NSCLC) and in 19 blood samples of healthy controls. Results Using t-test, we detected 27 miRNAs significantly deregulated in blood cells of lung cancer patients as compared to the controls. Some of these miRNAs were validated using qRT-PCR. To estimate the value of each deregulated miRNA, we grouped all miRNAs according to their diagnostic information that was measured by Mutual Information. Using a subset of 24 miRNAs, a radial basis function Support Vector Machine allowed for discriminating between blood cellsamples of tumor patients and controls with an accuracy of 95.4% [94.9%-95.9%], a specificity of 98.1% [97.3%-98.8%], and a sensitivity of 92.5% [91.8%-92.5%]. Conclusion Our findings support the idea that neoplasia may lead to a deregulation of miRNA expression in blood cells of cancer patients compared to blood cells of healthy individuals. Furthermore, we provide evidence that miRNA patterns can be used to detect human cancers from blood cells. -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 59.112.212.26